model ‘one site—fit total and nonspecific binding  (GraphPad Software Inc)

Journal: Nucleic Acids Research

Article Title: Aptamer binding footprints discriminate α-synuclein fibrillar polymorphs from different synucleinopathies

doi: 10.1093/nar/gkae544

Figure Lengend Snippet: Binding of F30 and F124 2′F-Py RNA aptamers on different F-type and R-type α-Syn fibrillar polymorphs compared to DNA aptamers previously described in the literature. ( A ) example of binding curves of F30 and F124 against F-type (upper panel) and R-type (lower panel) α-Syn fibrillar polymorphs. The binding of aptamers are in red while the binding of a scramble sequence was used to evaluate the nonspecific binding (black dotted curves). The binding curves were fit using the model ‘One site - Fit total and nonspecific binding’ from GraphPad Prism 9 to calculate the apparent K d of the interaction. ( B ) Apparent K d s of 2′F-Py RNA and DNA aptamers on different F-type and R-type α-Syn fibrillar polymorphs. The values presented in the table represent the average of the apparent K d s and their standard deviation calculated from completely independent experiments (in triplicate and duplicate for 2′F-Py RNA and DNA aptamers, respectively).

Article Snippet: The apparent K d values were determined by fitting binding curves using the model ‘One site—Fit total and nonspecific binding’ from GraphPad Prism 10 considering the binding of the scramble sequence as non-specific binding.

Techniques: Binding Assay, Sequencing, Standard Deviation